Cytidine-to-Uridine RNA editing factor NbMORF8 negatively regulates plant immunity to Phytophthora pathogens.
Identifieur interne : 000190 ( Main/Exploration ); précédent : 000189; suivant : 000191Cytidine-to-Uridine RNA editing factor NbMORF8 negatively regulates plant immunity to Phytophthora pathogens.
Auteurs : Yang Yang ; Guangjin Fan ; Yan Zhao ; Qujiang Wen ; Peng Wu ; Yuling Meng ; Weixing Shan [République populaire de Chine]Source :
- Plant physiology [ 1532-2548 ] ; 2020.
Abstract
Mitochondria and chloroplasts play key roles in plant-pathogen interactions. Cytidine-to-uridine (C-to-U) RNA editing is a critical post-transcriptional modification in mitochondria and chloroplasts that is specific to flowering plants. Multiple organellar RNA editing factors (MORFs) form a protein family that participates in C-to-U RNA editing, but little is known regarding their immune functions. Here, we report the identification of NbMORF8, a negative regulator of plant immunity to Phytophthora pathogens. Using virus-induced gene silencing (VIGS) and transient expression in Nicotiana benthamiana, we show that NbMORF8 functions through regulation of ROS production, SA signaling, and accumulation of multiple RXLR effectors of Phytophthora pathogens. NbMORF8 is localized to mitochondria and chloroplasts, and its immune function requires mitochondrial targeting. The conserved MORF box domain is not required for its immune function. Furthermore, we show that the preferentially mitochondrial localized NbMORF proteins negatively regulate plant resistance against Phytophthora, whereas the preferentially chloroplast localized ones are positive immune regulators. Our study reveals that the C-to-U RNA editing factor NbMORF8 negatively regulates plant immunity to the oomycete pathogen Phytophthora, and that mitochondrial and chloroplast localized NbMORF family members exert opposing effects on immune regulation.
DOI: 10.1104/pp.20.00458
PubMed: 32972981
Affiliations:
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first="Yan" last="Zhao">Yan Zhao</name><affiliation><nlm:affiliation>Northwest A&F University CITY: Yangling STATE: Shaanxi China [CN].</nlm:affiliation><wicri:noCountry code="no comma">Northwest A&F University CITY: Yangling STATE: Shaanxi China [CN].</wicri:noCountry></affiliation></author><author><name sortKey="Wen, Qujiang" sort="Wen, Qujiang" uniqKey="Wen Q" first="Qujiang" last="Wen">Qujiang Wen</name><affiliation><nlm:affiliation>Northwest A&F University CITY: Yangling STATE: Shaanxi China [CN].</nlm:affiliation><wicri:noCountry code="no comma">Northwest A&F University CITY: Yangling STATE: Shaanxi China [CN].</wicri:noCountry></affiliation></author><author><name sortKey="Wu, Peng" sort="Wu, Peng" uniqKey="Wu P" first="Peng" last="Wu">Peng Wu</name><affiliation><nlm:affiliation>Northwest A&F University CITY: Yangling STATE: Shaanxi China [CN].</nlm:affiliation><wicri:noCountry code="no comma">Northwest A&F University CITY: Yangling STATE: Shaanxi China [CN].</wicri:noCountry></affiliation></author><author><name sortKey="Meng, Yuling" sort="Meng, Yuling" uniqKey="Meng Y" first="Yuling" last="Meng">Yuling Meng</name><affiliation><nlm:affiliation>Northwest A&F University CITY: Yangling STATE: Shaanxi China [CN].</nlm:affiliation><wicri:noCountry code="no comma">Northwest A&F University CITY: Yangling STATE: Shaanxi China [CN].</wicri:noCountry></affiliation></author><author><name sortKey="Shan, Weixing" sort="Shan, Weixing" uniqKey="Shan W" first="Weixing" last="Shan">Weixing Shan</name><affiliation wicri:level="1"><nlm:affiliation>Northwest A&F University CITY: Yangling STATE: Shaanxi POSTAL_CODE: 712100 China [CN] wxshan@nwafu.edu.cn.</nlm:affiliation><country wicri:rule="url">République populaire de Chine</country></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32972981</idno><idno type="pmid">32972981</idno><idno 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uniqKey="Fan G" first="Guangjin" last="Fan">Guangjin Fan</name><affiliation><nlm:affiliation>Northwest A&F University CITY: Yangling STATE: Shaanxi China [CN].</nlm:affiliation><wicri:noCountry code="no comma">Northwest A&F University CITY: Yangling STATE: Shaanxi China [CN].</wicri:noCountry></affiliation></author><author><name sortKey="Zhao, Yan" sort="Zhao, Yan" uniqKey="Zhao Y" first="Yan" last="Zhao">Yan Zhao</name><affiliation><nlm:affiliation>Northwest A&F University CITY: Yangling STATE: Shaanxi China [CN].</nlm:affiliation><wicri:noCountry code="no comma">Northwest A&F University CITY: Yangling STATE: Shaanxi China [CN].</wicri:noCountry></affiliation></author><author><name sortKey="Wen, Qujiang" sort="Wen, Qujiang" uniqKey="Wen Q" first="Qujiang" last="Wen">Qujiang Wen</name><affiliation><nlm:affiliation>Northwest A&F University CITY: Yangling STATE: Shaanxi China [CN].</nlm:affiliation><wicri:noCountry code="no comma">Northwest A&F University CITY: Yangling STATE: Shaanxi China [CN].</wicri:noCountry></affiliation></author><author><name sortKey="Wu, Peng" sort="Wu, Peng" uniqKey="Wu P" first="Peng" last="Wu">Peng Wu</name><affiliation><nlm:affiliation>Northwest A&F University CITY: Yangling STATE: Shaanxi China [CN].</nlm:affiliation><wicri:noCountry code="no comma">Northwest A&F University CITY: Yangling STATE: Shaanxi China [CN].</wicri:noCountry></affiliation></author><author><name sortKey="Meng, Yuling" sort="Meng, Yuling" uniqKey="Meng Y" first="Yuling" last="Meng">Yuling Meng</name><affiliation><nlm:affiliation>Northwest A&F University CITY: Yangling STATE: Shaanxi China [CN].</nlm:affiliation><wicri:noCountry code="no comma">Northwest A&F University CITY: Yangling STATE: Shaanxi China [CN].</wicri:noCountry></affiliation></author><author><name sortKey="Shan, Weixing" sort="Shan, Weixing" uniqKey="Shan W" first="Weixing" last="Shan">Weixing Shan</name><affiliation wicri:level="1"><nlm:affiliation>Northwest A&F University CITY: Yangling STATE: Shaanxi POSTAL_CODE: 712100 China [CN] wxshan@nwafu.edu.cn.</nlm:affiliation><country wicri:rule="url">République populaire de Chine</country></affiliation></author></analytic><series><title level="j">Plant physiology</title><idno type="eISSN">1532-2548</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Mitochondria and chloroplasts play key roles in plant-pathogen interactions. Cytidine-to-uridine (C-to-U) RNA editing is a critical post-transcriptional modification in mitochondria and chloroplasts that is specific to flowering plants. Multiple organellar RNA editing factors (MORFs) form a protein family that participates in C-to-U RNA editing, but little is known regarding their immune functions. Here, we report the identification of NbMORF8, a negative regulator of plant immunity to Phytophthora pathogens. Using virus-induced gene silencing (VIGS) and transient expression in Nicotiana benthamiana, we show that NbMORF8 functions through regulation of ROS production, SA signaling, and accumulation of multiple RXLR effectors of Phytophthora pathogens. NbMORF8 is localized to mitochondria and chloroplasts, and its immune function requires mitochondrial targeting. The conserved MORF box domain is not required for its immune function. Furthermore, we show that the preferentially mitochondrial localized NbMORF proteins negatively regulate plant resistance against Phytophthora, whereas the preferentially chloroplast localized ones are positive immune regulators. Our study reveals that the C-to-U RNA editing factor NbMORF8 negatively regulates plant immunity to the oomycete pathogen Phytophthora, and that mitochondrial and chloroplast localized NbMORF family members exert opposing effects on immune regulation.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">32972981</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>25</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1532-2548</ISSN><JournalIssue CitedMedium="Internet"><PubDate><Year>2020</Year><Month>Sep</Month><Day>24</Day></PubDate></JournalIssue><Title>Plant physiology</Title><ISOAbbreviation>Plant Physiol</ISOAbbreviation></Journal><ArticleTitle>Cytidine-to-Uridine RNA editing factor NbMORF8 negatively regulates plant immunity to Phytophthora pathogens.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">pp.00458.2020</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1104/pp.20.00458</ELocationID><Abstract><AbstractText>Mitochondria and chloroplasts play key roles in plant-pathogen interactions. Cytidine-to-uridine (C-to-U) RNA editing is a critical post-transcriptional modification in mitochondria and chloroplasts that is specific to flowering plants. Multiple organellar RNA editing factors (MORFs) form a protein family that participates in C-to-U RNA editing, but little is known regarding their immune functions. Here, we report the identification of NbMORF8, a negative regulator of plant immunity to Phytophthora pathogens. Using virus-induced gene silencing (VIGS) and transient expression in Nicotiana benthamiana, we show that NbMORF8 functions through regulation of ROS production, SA signaling, and accumulation of multiple RXLR effectors of Phytophthora pathogens. NbMORF8 is localized to mitochondria and chloroplasts, and its immune function requires mitochondrial targeting. The conserved MORF box domain is not required for its immune function. Furthermore, we show that the preferentially mitochondrial localized NbMORF proteins negatively regulate plant resistance against Phytophthora, whereas the preferentially chloroplast localized ones are positive immune regulators. Our study reveals that the C-to-U RNA editing factor NbMORF8 negatively regulates plant immunity to the oomycete pathogen Phytophthora, and that mitochondrial and chloroplast localized NbMORF family members exert opposing effects on immune regulation.</AbstractText><CopyrightInformation>{copyright, serif} 2020 American Society of Plant Biologists. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Yang</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Northwest A&F University CITY: Yangling STATE: Shaanxi China [CN].</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fan</LastName><ForeName>Guangjin</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Northwest A&F University CITY: Yangling STATE: Shaanxi China [CN].</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Yan</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Northwest A&F University CITY: Yangling STATE: Shaanxi China [CN].</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wen</LastName><ForeName>Qujiang</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>Northwest A&F University CITY: Yangling STATE: Shaanxi China [CN].</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Peng</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Northwest A&F University CITY: Yangling STATE: Shaanxi China [CN].</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Meng</LastName><ForeName>Yuling</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Northwest A&F University CITY: Yangling STATE: Shaanxi China [CN].</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shan</LastName><ForeName>Weixing</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Northwest A&F University CITY: Yangling STATE: Shaanxi POSTAL_CODE: 712100 China [CN] wxshan@nwafu.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>09</Month><Day>24</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Physiol</MedlineTA><NlmUniqueID>0401224</NlmUniqueID><ISSNLinking>0032-0889</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>09</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>04</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>9</Month><Day>25</Day><Hour>5</Hour><Minute>53</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>9</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>9</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32972981</ArticleId><ArticleId IdType="pii">pp.20.00458</ArticleId><ArticleId IdType="doi">10.1104/pp.20.00458</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Fan, Guangjin" sort="Fan, Guangjin" uniqKey="Fan G" first="Guangjin" last="Fan">Guangjin Fan</name><name sortKey="Meng, Yuling" sort="Meng, Yuling" uniqKey="Meng Y" first="Yuling" last="Meng">Yuling Meng</name><name sortKey="Wen, Qujiang" sort="Wen, Qujiang" uniqKey="Wen Q" first="Qujiang" last="Wen">Qujiang Wen</name><name sortKey="Wu, Peng" sort="Wu, Peng" uniqKey="Wu P" first="Peng" last="Wu">Peng Wu</name><name sortKey="Yang, Yang" sort="Yang, Yang" uniqKey="Yang Y" first="Yang" last="Yang">Yang Yang</name><name sortKey="Zhao, Yan" sort="Zhao, Yan" uniqKey="Zhao Y" first="Yan" last="Zhao">Yan Zhao</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Shan, Weixing" sort="Shan, Weixing" uniqKey="Shan W" first="Weixing" last="Shan">Weixing Shan</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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